DNA sequences

Monday, September 17, 2007 - 3:00pm - 4:00pm
Stephen Levene (University of Texas at Dallas)
The formation of DNA loops by proteins bound at distant sites along a single
molecule is an essential mechanistic aspect of many biological processes
including gene regulation, DNA replication, and recombination. The
biological importance of DNA loop formation is underscored by an abundance
of architectural proteins in cells such as HU, IHF, and HMGs, which
facilitate looping by bending the intervening DNA between cognate
protein-binding sites. We have developed a rigorous theory for DNA loop
Monday, September 17, 2007 - 10:30am - 11:30am
Alexander Vologodskii (New York University)
DNA double helix should experience local breaks under sufficient bending or/and unwinding torsional stress. Local distortions of DNA under negative torsional stress have been studied in details, but until now very little has been known about distortions by bending stress. We addressed this question in the current study by probing the structure of very small DNA circles. First, we developed an efficient method to obtain covalently closed DNA minicircles. To detect breaks of regular DNA structure in these minicircles we treated them by single strand-specific endonucleases.
Sunday, September 16, 2007 - 10:45am - 11:45am
David Lilley (University of Dundee)
Genetic recombination is important in the repair of double strand DNA breaks, the processing of stalled replication forks and in the generation of genetic diversity in evolution. During this process, two homologous DNA molecules undergo strand exchange to form a four-way DNA (Holliday) junction. The junction adopts a folded structure in the presence of divalent metal ions, generated by pairwise coaxial stacking of helical arms.


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