Calcium imaging

Monday, April 23, 2018 - 10:30am - 11:00am
Daniela Witten (University of Washington)
In recent years, new technologies in neuroscience have made it possible to measure the activities of large numbers of neurons simultaneously in behaving animals. For each neuron, a fluorescence trace is measured; this can be seen as a first-order approximation of the neuron's activity over time. Determining the exact time at which a neuron spikes on the basis of its fluorescence trace is an important open problem in the field of computational neuroscience.
Thursday, February 15, 2018 - 1:30pm - 2:30pm
Theoden Netoff (University of Minnesota, Twin Cities)
The dynamics of spreading depolarizations (SD) operate at both the single cell and network level. As single cells bifurcate into extreme regions of their state space, network effects are all that remain to hold back the forthcoming wave of depolarization. Therefore, we investigated excitatory/inhibitory network effects near the leading edge of spreading depolarizations. We imaged >1000 layer 2/3 pyramidal neurons in vivo with GCaMP6f in awake mice before, during and after spreading depolarizations.
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