Wave Based Coherent 3D Microscopy

Friday, October 21, 2005 - 11:40am - 12:30pm
EE/CS 3-180
Anthony Devaney (Northeastern University)
A new form of coherent optical microscopy is described that
generates images (reconstructions) of two and three-dimensional,
penetrable scattering
objects computationally from sets of measured digital holograms
of scattered field data collected in a suite of scattering
experiments. The
microscope uses the technique of phase shifting holography
(explained in
the talk) to compute both the amplitude and phase of the
scattered field data which is then input into wave based
inversion algorithms
to generate the image of the object. For 2D (thin) objects the
inversion algorithm is based on a coherent back propagation of
field data
collected in single experiment and, unlike conventional
microscopy, yields
both the amplitude as well as phase of the 2D sample. For thick
objects the object is embedded in an index matching fluid and a
of scattering experiments is required using dirent incident
waves. In this case the object reconstruction is generated
using a generalized
filtered back propagation (FBP) algorithm that allows for
scattering between the object and index matching bath. The
FBP algorithm is described and examples using simulated and
real data
are presented.
MSC Code: