This thresholding method is shown below on an actual barcode derivative signal (sig2a5cm_8). The first plot shows the original derivative signal. The second plot is a stem plot of the signal after selective sampling. Note the size of the signal has been reduced by a factor of 6. The third plot is the histogram of the peak strengths. The red vertical line is the threshold found by Otsu's Algorithm at 0.84357. The red lines on the fourth plot show the thresholds at +/- 0.84357. As can be seen in the last plot, the method failed to produce alternating spikes throughout. The pictures were produced by the program otsu.m with the calls "load sig2a5cm_8.txt" and "[s,u] = otsu (sig2a5cm_8')". Shellhammer et. al describe in the paper how to incorporate the Gaussian mentioned in the previous section.

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