Slide 1

Outline of the Talk

Three “Laws”

Basic Facts

The Use of Clusters to Find
Missing genes

Central Machinery of Life:
Horizons of gene discovery

Missing genes in metabolic pathways
 making a case

Missing genes in metabolic pathways
 making a case

Techniques of genome context analysis (I)
 checking neighbors

Techniques of genome context analysis (II)
 checking connections

Techniques of genome context analysis (III)
 co-regulation

Techniques of genome context analysis (IV)
 co-evolution

Missing gene case
 primary suspects

Example I: Chorismate Pathway

Chromosomal Clustering: Prediction

Slide 16

Example II: “Missing Drug Target” in S.pneumoniae

Clustering of FAB Genes : Prediction

Slide 19

Missing genes, examples in cofactor pathways
prediction and experimental verification

The Leucine Degradation Cluster:
Origin of a New Perspective on
Uses of Clusters

Context-based enrichment of initial functional assignments
example from Brucella melitensis genome analysis

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Gene cluster in B. subtilis

Leucine degradation in Baccili

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The Project: Annotate 1000 Genomes in
Three Years

Annotation by Subsystem

FIG: Building the Initial Annotation Tools