Abstracts and Talk Materials:December 07-11, 2009 Shahriar Afkhami (New Jersey Institute of Technology)
http://web.njit.edu/~shahriar/ Numerical simulations of dynamic wetting
December 07, 2009 4:00 pm - 6:00 pm With miniaturization of fluidic devices, small-scale effects such as the
details of the flow near the contact line become important. We present a
three-dimensional numerical model to simulate the dynamic behavior of
moving contact line phenomena. The model consists of an adaptive mesh
discretization of the time-dependent Navier-Stokes equations for
incompressible two-phase flows with a volume-of-fluid technique for
interface tracking. Equilibrium results of three-dimensional droplets with
various contact angles are presented and compared with known solutions.
The slip of a moving contact line on the solid surface and the dynamical
contact angle are computationally investigated. Some numerical simulations
of the model applied to electrowetting are presented. Shelley L. Anna (Carnegie Mellon University)
http://www.me.cmu.edu/default.aspx?id=anna Scaling arguments for tipstreaming of submicron droplets
December 07, 2009 4:00 pm - 6:00 pm Microfluidic devices are convenient for producing highly uniform droplets for precise emulsions and lab-on-a-chip devices. However, the minimum droplet size in a microfluidic process is determined by the smallest geometric feature size, typically on the order of tens of microns. Introducing additional physico-chemical effects can help overcome this fundamental limitation. For example, when dissolved surfactants are present in one of the liquid phases, a tipstreaming-like phenomenon occurs, leading to the formation of submicron droplets. We have characterized this phenomenon in detail as a function of fluid properties and flow kinematics. However, we still have only a phenomenological understanding of the role of surfactant in the tipstreaming process. Experiments and recent literature suggest that the adsorption and desorption of surfactants at the interface plays an important role. In this poster, we demonstrate the feasibility of this hypothesis via scaling arguments for the diffusion, adsorption, and desorption of soluble surfactants in micron-scale geometries. Hsueh-Chia Chang (University of Notre Dame)
http://www.nd.edu/~changlab/ Electrokinetic ion transport and liquid flux across
nanochannels
December 09, 2009 3:20 pm - 4:00 pm Keywords: electrokinetics, nanoscience, limiting current, Donnan potential, ion selectivity, Warburg Impedance Abstract: With the advent of nanofabrication technologies,
nano-channels with dimensions smaller than the Debye screening
layer can now be fabricated to allow scrutiny of the various
anomalous DC and AC I-V characteristics of ion-selective
membranes at the single-pore level — such knowledge is
essential for rapid DNA sequencing, single-molecule
sensing/identification and plasmonic imaging in nanoscience.
Combining theoretical analyses of the underlying ion/solvent
fluxes and confocal imaging of velocity and ion concentration
fields, we explore the fundamental mechanisms behind non-ideal
selectivity, Donnan potential, asymmetric depletion/enrichment
layer formation, limiting and overlimiting-current, diode-like
rectification, Warburg impedance response, inter-channel
communication etc. Curiously, hydrodynamic effects at the
depletion end of the channel is found to control many of the
non-Ohmic behavior at higher voltages. Interfacial vortices
created by an osmotic pressure driven instability (first
predicted by I. Rubinstein) and induced charges at the corners
of nanopores are found to specify the overlimiting current, the
rectification factor and inter-pore communication. The
intensity of these vortices and their influence on the
ion-carried currents are found to be strongly dependent on the
pore/reservoir geometries and can be described by limiting
fundamental solutions of the Laplace and Stokes equations due
to severe electric and flow field focusing into the
nanochannel. Kevin D. Dorfman (University of Minnesota)
http://www.cems.umn.edu/research/dorfman DNA electrophoresis in microfabricated arrays
December 07, 2009 9:40 am - 10:20 am Keywords: Brownian dynamics, microfabrication, boundary element method, DNA electrophoresis, separations Abstract: I will present our recent results on the dynamics of long DNA as they move through a hexagonal arrays of microfabricated posts under the influence of an electric field. The first part of the talk focuses on the potential of using sparse, ordered post arrays to separate long DNA by molecular weight. In particular, I will focus on the crucial role of electric field gradients on the separation process and how we can use microfabrication tools to control these gradients. In the second part of the talk, I will demonstrate how we are using a combination of Brownian dynamics simulations and videomicroscopy to gain a detailed understanding of the separation process at the macromolecular level. Patrick S. Doyle (Massachusetts Institute of Technology)
http://web.mit.edu/doylegroup/ DNA dynamics in confinement and complex electric fields
December 08, 2009 1:30 pm - 2:10 pm Keywords: DNA, microfluidic, nanofluidic, electrophoresis Abstract: Large double stranded DNA are both a powerful system to study polymer dynamics at the single molecule level and also important molecules for genomic applications. While homogenous electric fields are routinely used to separate DNA in gels, DNA deformation in more complex fields has been less widely studied. We will demonstrate how micro/nanofluidic devices allow for the generation of electric fields with well-defined kinematics for trapping, stretching and then watching DNA relax back to equilibrium. The dimensions of the devices highly confine DNA and subsequently change both their conformation and dynamics. First, I will discuss how confinement changes the conformational relaxation time and introduces new relaxation regimes not seen in bulk. Next, I will show how these confinements effects change the coil-stretch transition of a DNA being electrophoretically stretched in a purely elongational electrical field. Jens Eggers (University of Bristol)
http://www.maths.bris.ac.uk/~majge/ Making small things
December 08, 2009 9:40 am - 10:20 am Keywords:
Singularities, Free surface flows Abstract: Viscous flow is extremely effective in deforming a free
surface into very sharp features such as tips or cups. Under increased
driving such free surface singularities may turn unstable, and
give way to secondary structures. In particular, this may be turned
into a method to "manufacture" small things by using the nonlinear
character of the equations of hydrodynamics. Thomas B. Jones (University of Rochester)
http://www.ece.rochester.edu/~jones/ Dielectrophoretic deflection and rebound of continuous droplet
streams
December 07, 2009 4:00 pm - 6:00 pm Joint work with Paul Chiarot (Department of Electrical and Computer Engineering, University of Rochester). In continuous ink jet systems, streams of ~10 picoliter liquid droplets (diameter ~30 microns) are ejected from an array of orifices at rates of up to 350,000 per second and velocities in excess of 20 m/s. Applications as diverse as printing, microfabrication, and microarraying benefit from this technology; however, reliable manipulation of the jet, including basic on/off control and steering of droplet streams and individual liquid droplets, remains difficult to achieve. We have developed a novel deflection scheme to manipulate the trajectories of droplets rebounding at shallow angles from a solid substrate based on the dielectrophoretic force exerted by patterned electrodes. Droplet rebound, key to the performance of this scheme, has been investigated for both fluorocarbon (Teflon) and superhydrophobic surface coatings. Our experiments reveal interesting droplet behavior, and at least two regimes of operation, that are dependent on the Weber number and on the properties of the solid surface with which the droplets collide and rebound.This work was supported by a grant from Eastman Kodak Co. in Rochester, NY (USA). Thomas B. Jones (University of Rochester)
http://www.ece.rochester.edu/~jones/ The electromechanics of liquids
December 09, 2009 10:50 am - 11:30 am Keywords: dielectrophoresis, electrowetting, electromechanics, microfluidics Abstract: When subjected to electric fields, liquids exhibit a large range of dynamic and kinematic phenomena. This lecture focuses on the liquid electromechanical effects commonly exploited to dispense, move, and manipulate small liquid masses (including droplets) in microfluidic applications. Irrespective of the fine details, the net, observable force interaction very often can be predicted in terms of an appealingly simple, lumped parameter model. In particular, as long as the capacitance of a microfluidic structure can be expressed in terms of a small number of mechanical variables that adequately describe liquid displacement and distortion, then an electromechanical system model is born. Because such models can describe the important fluid behavior of insulating and conductive liquids, microfluidic schemes based on either liquid dielectrophoresis, electrowetting-on-dielectric, or a combination of the two can be treated. The lumped parameter approach avoids issues associated with volume force densities and often circumvents the need for numerical electric field computations. Aditya Satish Khair (University of California, Santa Barbara)
http://engineering.ucsb.edu/~akhair/ Influence of ion sterics and hydrodynamic slip on
electrophoresis of a colloidal particle
December 07, 2009 4:00 pm - 6:00 pm The classical theory of a spherical colloids' electrophoretic mobility is founded on the Poisson-Nernst-Planck (PNP) equations and assumes the standard hydrodynamic no-slip boundary condition at the fluid/solid interface. In the (common) limit of thin double-layers, the mobility has long been known to exhibit a maximum at some zeta potential, then decrease and asymptote to a constant value. Dukhin, O'Brien, White and others showed this to result from the importance of excess ionic surface conductivity within the double-layer. The fundamental assumptions that underpin this result are, however, subject to challenge: in recent years, a finite liquid/solid slip has been measured over a variety of surfaces, and the PNP equations predict physically impossible ion concentrations precisely at the high zeta potentials where the mobility maximum occurs. Here, we discuss the dramatic effect that hydrodynamic slip and finite-ion-size steric effects in double-layers have upon the electrophoretic mobility of spherical colloids, and therefore upon the interpretation of electrophoretic mobility measurements. Reinhard Lipowsky (Max Planck Institute for Colloids and Interfaces)
http://www.mpikg.mpg.de/rl/ Wetting of structured substrates and flexible membranes
December 07, 2009 1:30 pm - 2:10 pm Keywords: Wetting phenomena, surface domains, surface topography,
contact line pinning, fluid membranes, vesicles, intrinsic contact angles Abstract: Two types of wetting phenomena will be discussed: (i) Morphological wetting
transitions at chemically patterned or topographically structured substrates; and
(ii) Wetting of flexible membranes such as lipid bilayers by aqueous phases.
Morphological wetting transitions between different droplet shapes occur, e.g., as one
varies the amount of liquid deposited on the structured substrate. [1,2] The basic
mechanism underlying this polymorphism is the freedom of contact angles at pinned
contact lines. [3] The second system consists of lipid vesicles containing aqueous
solutions with two species of water-soluble polymers. When the polymer concentrations
are raised by deflation, the aqueous solution forms two coexisting liquid phases that
may undergo complete-to-partial wetting transitions at the vesicle membranes. [4] Partial
wetting is characterized by effective contact angles that can be measured by optical
microscopy and by an intrinsic contact angle that represents a 'hidden' material parameter. [5][1] R. Seemann et al. PNAS 102, 1848 (2005)
[2] P. Blecua, M. Brinkmann, R. Lipowsky, and J. Kierfeld. Langmuir (in press)
[3] R. Lipowsky et al. J. Phys.: Condens. Matter 17, S2885 (2005)
[4] Y.-H. Li, R. Lipowsky, and R. Dimova. JACS 130, 12252 (2008)
[5] H. Kusumaatmaja, Y.-H. Li, R. Dimova, and R. Lipowsky. Phys. Rev. Lett. (submitted) Chun Liu (University of Minnesota)
http://www.math.psu.edu/liu/ Energetic variational approaches in calcium and sodium
channels
December 09, 2009 2:10 pm - 2:50 pm Keywords: Energetic Variational Approaches, ion channels, complex fluids Abstract: Ion channels are key components in a wide variety of biological processes, such as nerve impulse,
cardiac and muscle contraction, regulating the secretion of hormones into the bloodstream.
Ion channels are a frequent target in the search of new drugs.Ion channels, like enzymes, have their specific properties: potassium, sodium, calcium, and chloride
channels allow only that type of ions to move through the pores. This selectivity is the key to
all those biological process mentioned above.Selectivities in both calcium and sodium channels can be described
by the reduced models, taking into consideration of
dielectric coefficient and ion particle sizes, as well as their
very different primary structure and properties.
The side-chains are represented only as charged spheres
(calcium channel EEEA/EEEE; sodium channel DEKA).
These self-organized systems will be modeled and analyzed
with energetic variational approaches (EnVarA)
that were motivated by classical works of Rayleigh and Onsager.
The resulting/derived multiphysics-multiscale systems automatically satisfy
the Second Laws of Thermodynamics and the basic physics that are involved in the system,
such as the microscopic diffusion, the electrostatics
and the macroscopic conservation of momentum, as well as the physical boundary conditions.In this talk, I will discuss the some of the related biological, physics, chemistry and mathematical issues.This is a joint work with Bob Eisenberg (Rush) and Yunkyong Hyon (IMA). Detlef Lohse (Universiteit Twente)
http://pof.tnw.utwente.nl Hydrodynamics challenges in inkjet printing
December 09, 2009 9:40 am - 10:20 am Keywords: inkjet printing, bubbles, drop formation, air entrainment, impact Abstract:
Piezo-acoustic inkjet printing has become a mature technique for high performance printing. Nevertheless, there are still various scientific challenges. In this overview talk I will cover some of them:
(i) Coupling between the fluid dynamics and the acoustics, in particular when a disturbing bubble has been entrained in the ink channel.
(ii) Optical and acoustical monitoring of the bubble.
(iii) Mechanisms of the bubble entrainment.
(iv) Droplet formation and pinch-off of droplets.
(v) Droplet impact on substrates.Main further contributors to the research: Jos de Jong, Roger Jeurissen, Arjan van der Bos, Michel Versluis, and the colleagues from Oce Technologies: Hans Reinten, Herman Wijshoff, and Marc van der Berg. Chaouqi Misbah (Université de Grenoble I (Joseph Fourier))
http://www-lsp.ujf-grenoble.fr/recherche/a3t2/a3t2a3/misbah/index.htm Vesicles and red blood cells under shear and Poiseuille flow
December 10, 2009 9:40 am - 10:20 am Keywords: Blood flow, microcirculation, modeling, rheology Abstract: Various rich dynamics of vesicles under linear and nonlinear
flows will be discussed. We present analytical and numerical
results on tank-treading motion, tumbling and
vacillating-breathing (aka swinging, trembling). We then
discuss the notion of transverse migration due to a wall and to
a nonlinear flow. We show theoretical and exeprimental results
on the law of transverse migration in a microfluidic device.
Finally, we present very recent results on a longstadning
puzzle of the blood microcirculatory research: why do red blood
cells adopt a non symmetric shape (called slipper) in small
blood vessels?A key result of our study is that the parachute symmetric
shape is shown to be unstable, while the slipper shape is
stable. That is, small flow disturbances–which are always
present in real blood flows–cause RBCs to assume slipper
shapes. It is further shown that the slipper shape offers a
better transport efficiency to RBCs. In addition the slipper
shape favors hemoglobin mixing in the cell, and thus enhances
oxygen transport efficiency. Blood flow efficiency together
with optimal oxygen supply seem to be determinant for natural
selection of slipper shapes. Frieder Mugele (Universiteit Twente)
http://pcf.tnw.utwente.nl/people/scientific_staff/mugele/ Surface charge measurement and control by gate voltage in
electroosmotic flow
December 07, 2009 4:00 pm - 6:00 pm We present a simple analytical model that allows for determining the
surface charge in electro-osmotic flow channels using the so-called
solution displacement method. In contrast to earlier techniques, which
have either been limited to small ratios of salt concentration or
required a numerical solution of the convection-diffusion equation, our
method provide a simple functional form with merely two fit parameters
and thus allow for more accurate measurements of surface charge.
Moreover, we demonstrate flow reversal inside our microfluidic channels
controlled by gate electrodes underneath insulating layers that allow
for external tuning of the surface charges. We discuss possible
applications as a rheometer for applying shear forces to ultrasoft
complex fluids. Frieder Mugele (Universiteit Twente)
http://pcf.tnw.utwente.nl/people/scientific_staff/mugele/ Mixing and internal flows in drops in AC electrowetting
December 07, 2009 2:10 pm - 2:50 pm Keywords: Electrowetting, mixing, electrothermal flow Abstract: Mixing is a key issue in microfluidics, including droplet-based “digital” microfluidics. In electrowetting, internal flow patterns inside drops can be generated without any lateral translation if the drops are excited with AC voltage. Two regimes can be distinguished: at AC frequencies of the order of the eigenfrequency (typically O(1kHz) or less), the drops periodically oscillate between states of high and low contact angle. Despite the periodicity, there is a symmetry breaking in the drop shape between the spreading and the receding phase, which causes a time-averaged net flow inside the drop that promotes mixing. This process can be described using a model based on capillary wave-driven Stokes drift. For somewhat higher frequencies, this mechanism becomes progressively inefficient, because the liquid cannot follow the applied voltage anymore. At substantially higher AC frequencies (typically >>10kHz, depending on the salt concentration), however, a new driving mechanism for internal flows sets in, as reported by Nichols and Gardniers (Anal. Chem. 79, 8699 (2007) and by Ko et al. (Langmuir 24, 1094, 2008). Under these conditions, the liquid no longer acts as a perfect conductor. The electric field penetrates into the drop and generates local Ohmic currents. These currents produce Joule heating and ultimately giving rise to electro-thermal flows inside the drops. Solving numerically for the distribution of the electrostatic field, the flow field, and the temperature distribution, we show that the flow velocity scales with the fourth power over a wide range of both the applied voltage and the AC frequency – in good agreement with the experiments by Ko et al. Monika Nitsche (University of New Mexico)
http://www.math.unm.edu/~nitsche/ High order quadratures for the evaluation of
interfacial velocities in axi-symmetric Stokes flows
December 07, 2009 4:00 pm - 6:00 pm Boundary integral methods are computationally efficient
in computing the evolution of interfaces in Stokes flow.
For axisymmetric interfaces, they reduce to evaluating a
1d integral at each time step. We have performed a detailed
analysis of the structure of the integrands and show that
standard methods of integration present two difficulties.
One arises from loss of precision due to cancellation, the
other from singular behaviour of the integrands near the
axis of symmetry. As a result, high order quadrature
proposed previously for these types of integrals are not
uniformly high order. Instead, the maximal errors are
always of second order. We propose a remedy to both
difficulties and present a uniformly accurate 5th order
approximation. This new quadrature is implemented to evolve
(1) an initially bar-belled bubble that pinches at a
point in finite time, and (2) a sphere in a strain flow
that approaches a steady state. We compare the results
with commonly used second order approximations and show
that significant improvement is obtained using 5th order
rules. The examples also illustrate when the corrections
needed for uniformity have an impact in practice. Peter D. Olmsted (University of Leeds)
http://www.irc.leeds.ac.uk/~phy6pdo/ The influence of boundaries on shear banding in complex fluids
December 11, 2009 10:50 am - 11:30 am Keywords: shear banding,
boundary conditions,
flow instabilities,
wormlike micelles Abstract: Shear banding fluids such as wormlike micelles, lamellar surfactant solutions, and emulsions, undergo "transitions" between fluids of different apparent viscosity as a function of applied flow conditions. This switching makes them appealing for use in microfluidic applications. Since their physics is controlled by the influence of flow on internal microstructure, which in turn is influenced by boundary effects, it is important to develop an understanding of how different kinds of boundary effects influence shear banding. In this talk I will discuss some of these issues, mainly using constitutive models inspired by experiments on wormlike micelles or polymer solutions. and explore the effects of different boundary conditions for the additional viscoelastic stress. These are needed because the equations of motion are inherently non-local and include “diffusive” or square-gradient terms. I will note some of the features expected to be important for microfluidic geometries.[Work done in collaboration with JM Adams and SM Fielding] Jonathan D. Posner (Arizona State University)
http://microfluidics.asu.edu/ Locomotion of synthetic nanomotors
December 07, 2009 4:00 pm - 6:00 pm At ASU, we are investigating locomotion of bimetallic synthetic nanomotors that, analogous to their biological counterparts, harvest chemical energy from their local environment and convert it to useful work. Bimetallic nanorods can autonomously propel themselves at a hundred body lengths per second through aqueous solutions by using hydrogen peroxide as a fuel. Magnetic fields and electrochemically induced chemical species are used to control the motion of Pt-Ni-Au nanorods. We use the magnetic properties of nickel-loaded nanomotors to control their motion through micron-scale structures as well as the loading, transport, and release of spherical cargo that have volumes two orders of magnitude larger than the nanomotors itself. Nanomotor locomotion forces are determined by measuring their velocity while towing spherical cargo that have Stokes drag eight times the nanomotors themselves. Several physical arguments have been proposed to describe the physics underlying chemically-powered locomotion, but there is no detailed theory on the propulsion mechanism. We are simulating the physics of rod-shaped nanoparticles with asymmetric surface fluxes. Our models show that locomotion is driven by electric body forces in the fluid that arise due to finite space charge and internally generated electric fields surrounding the rod. The electric fields and charge density are generated by dipolar cation fluxes, such as those generated by heterogeneous electrochemical reactions with broken symmetry. The scaling analysis and detailed simulations predict that the nanomotor velocity depends on the reaction flux, nanorod electrical surface potential, solvent viscosity, and rod geometry. David Quéré (École Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI)) Impact figures
December 09, 2009 9:00 am - 9:40 am Since Worthington, many situations generated by impacts in liquids were
documented and explored. In this spirit, we would like to present several
recent observations related to the behavior of projectiles after they hit
different kinds of liquids. We first discuss the impact on soap films, and
naturally extend these observations to foams, focussing on the ability of
such complex fluids to absorb the kinetic energy of the projectiles. We
also consider impacts in a viscous liquid, and describe the particular
kind of cavity generated by the shock. And we conclude by looking at the
behaviour of revolving projectiles, discussing the characteristics of the
trajectory induced by the rotation. Other contributors to this talk: Anne Le Goff, Guillaume Dupeux and
Christophe Clanet. Antonio Ramos (University of Sevilla)
http://grupo.us.es/ehd-cgm/miembros/Ramos/eng/index.html Traveling-wave electroosmosis and faradaic currents: the
diffusion layer
December 07, 2009 4:00 pm - 6:00 pm Pumping of electrolytes in microchannels can be achieved with arrays of microelectrodes subjected to AC potentials. Here we show experiments on electrolyte flow induced by microelectrodes subjected to traveling-wave potentials. For sufficiently high voltages, Faradaic currents are present, leading to changes in the liquid properties and, in particular, changes in pH. A remarkable feature of the observations is that at voltages above
a threshold, the direction of the fluid flow is reversed. These observations motivate the theoretical study of Faradaic currents in electrokinetics for the general case of ionic species with different mobilities. We find, using a linear analysis, that the structure of the electrical double layer (EDL) has to be extended. The EDL consists of the compact and Debye layers, as in previous models, plus a diffusion layer that arises as a consequence of Faradaic currents. For the general case of different mobilities, there is a net electrical charge associated to the diffusion layer. As a particular result of this model, we show that traveling potentials generate flow in the reverse direction for the case of a thick compact layer and facile Faradaic reactions, if the reacting ions are the more mobile. This situation is consistent with the experimental observation of changes in pH due to proton reactions at the electrodes. Isaak Rubinstein (Ben Gurion University of the Negev)
http://bidr.bgu.ac.il/bidr/research/staff/rubinshtein/index.htm Extended space charge effects in concentration polarization
December 10, 2009 1:30 pm - 2:10 pm Keywords:
Extended Space Charge, Concentration Polarization, Overlimiting Conductance, Nonequilibrium Electroosmotic Instability Abstract: Our talk is about ionic currents from an electrolyte solution into a charge selective solid, such as, an electrode, an ion exchange membrane or an array of nano-channels in a micro-fluidic system, and the related viscous fluid flows on the length scales varying from nanometers to millimeters. All systems of this kind have characteristic voltage-current curves with segments in which current nearly saturates at some plateau values due to concentration polarization – formation of solute concentration gradients under the passage of a DC current. We start by reviewing a few seemingly different phenomena occurring in that range. These are anomalous rectification in cathodic copper deposition from a copper sulfate solution, super-fast vortexes near an ion-exchange granule, over-limiting conductance in electrodialysis and the recently observed non equilibrium electroosmotic instability. All these phenomena result from formation of an additional extended space charge layer next to that of a classical electric double layer at the solid/liquid interface. We review the peculiar features the extended space charge and their relation to the mechanisms of the above mentioned phenomena. Riccardo Sacco (Politecnico di Milano)
http://www1.mate.polimi.it/~ricsac/ Multi-physics computational models for neuro-chip
simulation
December 07, 2009 4:00 pm - 6:00 pm
Neuro-chips (NCs) are bio-hybrid devices in which living brain cells
and silicon circuits are coupled together. NCs are presently being
used as a non-invasive technique to record cellular response to drugs,
and are expected to be used in the cure of neurological disorders
through the creation of sophisticated neural prostheses.
The main technological challenge in the design of NCs is the
efficient transduction of the input biological signal (ion current of
the order of nA) into an output signal (electrical current) which is
modulated by the effective driving voltage of the open Gate of
the silicon device (of the order of mV). In order to devise a
sound simulation tool of the I/O behavior of a NC device, we
propose a multi-physics computational model including:
1) the Poisson-Nernst-Planck system, to account for intracellular and
extracellular electrochemical ion transport;
2) the Hodgkin-Huxley system, to describe ion transport across
membrane channels;
3) a nonlinear MOS capacitor approximation, to account for
cell-to-chip coupling.
The nonlinear system arising from the coupled solution of
1)-3) is successively solved by a functional iteration procedure,
and for each time level of the simulation, each obtained
sub-problem is numerically solved using a stabilized mixed-hybridized
finite element discretization scheme.
In order to provide a successful validation of the computational
procedure, we discuss preliminary results on two cases of physiological
interest, namely, the Hodgkin-Huxley axon and the response of a
field-effect transistor with metal-free gate oxide under an
intracellular voltage depolarization stimulating impulse. Eric S. G. Shaqfeh (Stanford University)
http://antares.stanford.edu/ The microfluidics of colloidal particle-vesicle-capsule
mixtures with application to blood additives
December 08, 2009 10:50 am - 11:30 am Keywords: Brownian rods, surfboards, drug delivery, capsules, vesicles, margination Abstract: Many dispersions of colloidal particles with application in materials processing, biological assays, or medicine, contain elongated particles (e.g. ellipsoidal disks, rods, etc.) Recently these particles have been used in drug delivery applications because of the inability of leukocytes to easily rid them from the circulation. Moreover such particles are useful at the nanoscale for application in cancer therapies, either for detection of tumor vasculature or for the delivery of anti-cancer agents to tumor endothelial cells. Thus, the study of anisotropic particulate flows with adhesion in microchannels especially in mixtures with vesicle flows (i.e. red blood cells) has taken on a particularly important set of engineering applications. In order to understand the transport in these systems, a numerical simulation must include: a) a high fidelity representation of nonequilibrium dynamics of vesicles and capsules in microflows, b) the dynamic simulation of Brownian colloidal particles of general shape in microflows, and c) the combination of these in mixtures at finite concentration. Each of these transport processes brings in new physics which we will review. In discussing a) we will focus on the transition between tank-treading, tumbling, and trembling dynamics in flow and whether these transitions also happen at finite concentration in microfluidics. In b) we will discuss the particle concentration distribution of Brownian nonspherical particles in microfluidic flows and its relation to the nonequilibrium osmotic pressure. In c) we will examine how particle margination in mixtures occurs and how it is related to the concentration of vesicles/capsules in the microflow. Ultimately, we will look toward the virtual prototyping and engineering of these therapies. John Denis Sherwood (University of Cambridge)
http://www.damtp.cam.ac.uk/people/j.sherwood/ Electrical streaming potential generated by 2-phase flow
December 10, 2009 9:00 am - 9:40 am Keywords: Streaming potential, multiphase flow, porous media Abstract: Streaming potentials generated by single phase flow are reasonably well-understood, but much less is known about the effects of multiphase flow. The introduction of a second fuid phase can either increase the wall shear rate (thereby increasing streaming currents caused by convection of ionic charge clouds), or can decrease it, depending on the ratio of the viscosities of the two phases and on the effect of interfacial tension. If the second phase is non-conducting (e.g. oil droplets in water), the effective conductivity of the mixture is decreased, and streaming potentials tend to increase. I shall review the few experimental results that are available, and describe recent theoretical work aimed at understanding the effect of a single particle or fluid droplet flowing through a capillary.
J.D. Sherwood, Streaming potential generated by two-phase flow in a capillary. Phys. Fluids, 19 (2007) 053101.J.D. Sherwood, Streaming potential generated by a long viscous drop in a capillary. Langmuir, 24 (2008) 10011.E. Lac & J.D. Sherwood, Streaming potential generated by a drop moving along the centreline of a capillary. J. Fluid Mech. (in the press) Zuzanna S. Siwy (University of California, Irvine)
http://www.physics.uci.edu/~zsiwy/ Ion transport through nanopores: From living cells to
diodes and transistors
December 11, 2009 9:40 am - 10:20 am Keywords: nanofluidics, channel, diode, ion current Abstract: Transport through nanopores and ion channels exists in virtually all biological cells and is important in such things as the regulation of heart function, nerve signals, and delivery of nutrients to the cell. Nanopores have also started to play a major role in contemporary biotechnology, because many separation and sensing processes require pores with nanometer-sized openings. My scientific interests have been focused on fabricating synthetic single nanopores with applications in biophysics and nanotechnology. The nanopores that we fabricate by the track-etching technique have diameters as small as 1 nanometer, they have controlled geometry and surface chemistry. I will show application of these nanopores as devices for controlling the flow of ions and charged molecules in a solution, functioning as ionic bipolar and unipolar diodes as well as ionic transistors. These functions are achieved by patterning the surface charge of the pore walls. Our systems will be applicable in nanofluidic, lab-on-the-chip, and biosensing systems. An example for application of ionic diodes in building sensors for anthrax will be discussed. I will also show how to induce ion current oscillations in time with
frequencies between tens of Hz and fractions of Hz. Paul H. Steen (Cornell University)
http://www.cheme.cornell.edu/cheme/people/profile/index.cfm?netid=phs7 Countering capillarity with electrokinetics: from micro-manipulation to Debye-layer diagnostics
December 10, 2009 10:50 am - 11:30 am Electroosmosis, originating in the Debye-layer near the solid/liquid boundary within a fully-saturated porous substrate, can pump successfully against the capillary pressure arising from the surface tension of a droplet placed in series with the pump. As droplet size diminishes, the voltage required to pump electroosmotically down-scales favorably. The technological implication is that electromechanical transducers made of large arrays of small droplets, so-called 'droplet micro-manipulators', and Debye-layer 'diagnostic machines', inferring zeta-potential by measuring interface deflection of a droplet-pump-droplet configuration, become feasible. These applications will be illustrated and we will describe the current modeling of such multi-scale interfacial systems, highlighting open questions that might benefit from an applied mathematical approach. Ulrich Tallarek (Philipps-Universität Marburg)
http://www.uni-marburg.de/fb15/ag-tallarek Electric field gradient focusing in microchannels with embedded bipolar electrode
December 07, 2009 4:00 pm - 6:00 pm The complex interplay of electrophoretic, electroosmotic, bulk convective, and diffusive mass/charge transport in a hybrid poly(dimethylsiloxane) (PDMS)/glass microchannel with embedded floating electrode is analyzed. The thin floating electrode attached locally to the wall of the straight microchannel results in a redistribution of local field strength after the application of an external electric field. Together with faradaic reactions taking place at the bipolar electrode and buffer reactions, as well as bulk convection based on cathodic electroosmotic flow, an extended field gradient is formed in the anodic microchannel segment. It imparts a spatially dependent electrophoretic force on charged analytes and, in combination with the bulk convection, results in an electric field gradient focusing at analyte-specific positions. Analyte concentration in the enriched zone approaches a maximum value which is independent of its concentration in the supplying reservoirs. A simple approach is shown to unify the temporal behavior of the concentration factors under general conditions. Petia M. Vlahovska (Dartmouth College)
http://engineering.dartmouth.edu/faculty/regular/petiavlahovska.html Dynamics of drops and vesicles in electric fields
December 07, 2009 4:00 pm - 6:00 pm Drop deformation in uniform electric fields is a classic problem. The pioneering work of G.I.Taylor demonstrated that for weakly conducting media, the drop fluid undergoes a toroidal flow and the drop adopts a prolate or oblate spheroidal shape, the flow and shape being axisymmetrically aligned with the applied field. However, recent studies have revealed a nonaxisymmetric rotational mode for drops of lower conductivity than the surrounding medium, similar to the rotation of solid dielectric spheres observed by Quincke in the 19th century. I will present an experimental and theoretical study of this phenomenon in DC fields. The critical electric field, drop inclination angle, and rate of rotation are measured. For small, high viscosity drops, the threshold field strength is well approximated by the Quincke rotation criterion. Reducing the viscosity ratio shifts the onset for rotation to stronger fields. The drop inclination angle increases with field strength. The rotation rate is approximately given by the inverse Maxwell-Wagner polarization time. We also observe a hysteresis in the tilt angle for low-viscosity drops.I will also discuss our work on drops encapsulated by complex interfaces such as lipid bilayer membranes. A comparison between the behavior of drops and giant vesicles (cell-size lipid membrane sacs) highlights new features due to the membrane electromechanics.This work is in collaboration with Paul Salipante (Dartmouth) and Dr. Rumiana Dimova’s group (Max Planck Institute of Colloids and Interfaces). Ehud Yariv (Technion-Israel Institute of Technology)
http://www.technion.ac.il/~yarive/ Migration of ion-exchange particles under the action of a uniformly applied
electric field
December 08, 2009 9:00 am - 9:40 am An ideally polarizable cation-selective solid particle is suspended in an
electrolyte solution and is exposed to a uniformly applied ambient electric
field. The electrokinetic transport processes are described in a closed
mathematical model, consisting of differential equations, representing the
physical balance laws, as well as boundary conditions and integral constraints,
representing the physicochemical condition on the particle boundary and at
large
distances away from it. Solving this model would in principle provide the
electro-kinetic flow about the particle and the concomitant particle drift
relative to the otherwise quiescent fluid. Using matched asymptotic expansions, the model is analyzed in the
thin-Debye-layer limit. An effective `macroscale' description is extracted,
whereby effective boundary conditions represent appropriate asymptotic matching
with the Debye-scale fields. The macroscale description significantly differs
from that corresponding to a chemically inert ideally polarizable particle.
Thus, ion selectivity on the particle surface results in a macroscale salt
concentration polarization, whereby the electric potential is rendered
non-harmonic. Moreover, the uniform Dirichlet condition governing this
potential on the particle surface is transformed into a non-uniform Dirichlet
condition on the macroscale particle boundary. The Dukhin--Derjaguin slip
formula still holds, but with a non-uniform zeta potential that depends upon
the salt concentration distribution.For weakly applied fields, an approximate solution is obtained as a
perturbation
to an equilibrium state. The linearized solution corresponds to a uniform zeta
potential; it predicts a particle velocity which is proportional to the applied
field. The associated electrokinetic flow differs however from that in the
comparable electrophoresis of an inert particle surface, since it is driven by
two different agents, electric field and salinity gradients, which are of
comparable magnitude. The velocity field, specifically, is rotational. Minami Yoda (Georgia Institute of Technology) Nonuniform interfacial colloidal tracer distributions
and implications for microscale PIV
December 08, 2009 2:10 pm - 2:50 pm Keywords: Interfacial particle-image velocimetry, colloidal particles, microfluidics, electrokinetically driven flows Abstract: Interfacial effects are important in many cases for microscale transport. One of the few experimental techniques that can resolve interfacial transport with sub-micron spatial resolution is evanescent wave-based, or nano-, particle-image velocimetry (PIV), which determines fluid velocities over the first 500 nm next to the wall from the displacements of 100-500 nm neutrally buoyant tracers. The wall-normal spatial resolution of nano-PIV is further improved by multilayer nano-PIV (MnPIV), which exploits the exponentially decaying intensity of evanescent-wave illumination to obtain velocities at different distances from the fluid-solid interface within about 500 nm of the wall. In agreement with DLVO theory, the distribution of the colloidal tracers within a particle diameter of the wall measured by MnPIV is highly nonnuniform due to repulsive electric double layer interactions and van der Waals effects. Nevertheless, the MnPIV results for steady creeping Poiseuille flow are in good agreement with analytical predictions once the velocities have been corrected for this nonuniform distribution. This talk describes velocity and Brownian diffusion coefficient measurements obtained from tracers with diameters ranging from 100 nm to 500 nm for Poiseuille flow through hydrophilic and hydrophobically coated fused-silica microchannels and for electrokinetically driven flows through fused-silica microchannels with a minimum cross-sectional dimension of about 40 microns. The near-wall particle distributions for 500 nm tracers are shown to vary with electric field for the electrokinetically driven flows, due presumably to electrophoresis and induced charge electroosmosis. |
